Thushara Nethramangalath

3 Questions 3 Answers 0 Followers

Questions related from Thushara Nethramangalath

RNASEQ vs qRTPCR discrepancy in gene expression?

Hi, I recently did RNA seq for my samples previously having done qRTPCR for a select number of genes. However as I was analyzing the data I noticed a few discrepancies. In my sample for QRTPCR...

18 August 2024 2,085 5 View

Can we increase the amount of mouse Leukemia Inhibitory Factor (mLIF) to 2000U/ml in culture of stem cells?

Hi , I have been noticing increased differentiation in my mouse embryonic stem cell culture. We culture cells in both 15%FBS.KODMEM media as well as the the 2i /N2B27 media. For the serum...

06 November 2023 9,735 0 View

Blebbing or exosomes in mouse embryonic stem cells?

Hi, I pulled out mouse embryonic stem cells from -80 (where they have been for a week when I froze them down) and plated them on gelatin plates without feeders. The next day I observe these round...

17 May 2022 1,783 3 View