RNA with low RIN value?

14 December 2020 1 10K Report

I have recently extracted RNA from human tumours grown in mice - for most of the samples I have high RNA yield with high RIN values.

For some I have high RNA concentrations (~100ng/ul) with low RIN numbers (2-3). Is there anything I can do to repurify the RNA do get rid of the degraded sequences?

Norman Hafner

...in principle- yes. But I would not recommend this, because you do not know if the degradation is uniform through the transcriptom. Thus, the residual high molecular weight fraction may not represent the transcriptom before the degradation process happend :-(

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