Lymhpoma cell culture issues, help?

Hi! I need help for some issues in growing mantle cell lymphoma cell lines (REC-1, MAVER-1, Granta-519, Mino, Jeko-1). I noticed that it is a little bit hard to maintain a stable cell culture:...

09 February 2021 7,402 11 View

Help to set up an in vitro Bioaccessibility assay?

Hi everyone! i am looking for protocols to evaluate the bioaccessibility of carotenoids in plant matrix. Ideally the best would be a quite easy protocol that could be used massively in the lab,...

02 February 2021 5,516 2 View

How seminal was the influence of Giuseppe Campos Venuti and Federico Oliva in Italy, France, Spain and other countries?

The question is what remains in the urban planning and in our professional and intellectual experiences of Giuseppe Campos Venuti and Federico Oliva? What about discipline now? They reasoned and...

30 October 2020 5,450 4 View

Is Sytox (from Thermo Fisher Scientific) a good choice to dectect NETs in flow cytometry using whole blood?

I found several papers in the literature where Sytox (green, red, blue, or orange) is used to detect Neutrophil Extracellular Traps, but it's always used with purified neutrophils. Do you know if...

07 October 2020 5,865 2 View

HPLC Alternative for a new project?

dear collegues, i would like to know if there are any high throughput and specific techniques alternative to the HPLC analysis for protein detection and if possible their relative costs. Thank...

04 October 2020 9,928 3 View

Maximum concentration for HPLC???

Dear Collegues, I'm starting a new project and I need to know what is the maximum concentration for a sample that have to be processed by analytical HPLC. Thank you so much for the answers! Luca

01 October 2020 1,654 5 View

How to make ethyl cellulose water soluble?

Good evening, I'm looking for a way to make ethyl cellulose soluble in water. I read about a low ethoxylated formulation with a DS around 1-1,5 but I cannot find it in any shop. Is there anyone...

20 July 2020 8,592 1 View

Single cell RNA sequencing on immune cells infiltrated in tissues: what is the best way?

I am planning to investigate how immune cells infiltrated in certain tissues may change between different mouse models. If I don't want to focus on a specific immune cell type, is it possible to...

30 June 2020 1,834 3 View

Empatica E4 data processing tools and strategies?

Hello there! I am a Masters student working at EPFL with Empatica E4. For the moment, as both the device and the data from it are very new to me, I am testing the solidity of the signals. At first...

14 June 2020 6,185 2 View

Binary Classification - Machine Learning - When using which one?

Hey, I don't exactly understand how I make the decision to choose a SVM or a DNN or Linear Classifier for my binary classification problem. Are there any aspects to proove and then decide? Or is...

28 April 2020 4,728 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View