Hello,
For the extraction of S. mutans RNA, I used the RNeasy Mini Kit Qiagen protocol. Just after the TRIS-HCl + EDTA + Proteinase K + Lysozyme step, then RLT Buffer + B-mercapto, I passed the sample into glass beads tubes (0.3g) and vortexed for 15 min, but I found small amounts of RNA. I did another test adding the mutanolysin and I vortexed the glass beads tubes (0.8g) for 1H and there the cells are still intact and I found small amounts of RNA for biofilms and planktonic.
Is there another reliable protocol for extracting S. mutans RNA?
Thank you
Thank you Nagendra Nath Das
,Indeed, I referred to the paper "Extraction and purification of total RNA from Streptococcus mutans biofilms" written by the same author. I have applied the sonication process but it did not work. I always obtain 80 ng/µl =< RNA quantity =< 220 ng/µl.
Dear Mariem
Try this article link
Article Isolation and purification of total RNA from Streptococcus m...
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