I am looking for nucleotide sequence information for binding sites of ribosomal proteins (on ribosomal RNA) in eukaryotes. For some reason all I find is protein amino acid binding motifs.
I am intrerested in modern interiors, history of them, design understanding. I would like to study about the modernization processes and changing interiors, how they were affected by the...
29 March 2020 6,537 7 View
Hi Fused silica bonding methods mostly requires high temperature (800+ ° C), however kapton becomes unstable at temperatures higher than 450° C. The device needs to be transparent and requires...
14 October 2018 6,276 2 View
I'm trying to apply the method of liu-liang 2010 [1]. They use potential temperature difference between 20mb and 5mb above surface and a threshold of 1K for land and 0.2K for ocean and ice. But in...
08 November 2016 667 2 View
I followed the manual for Arlequin 3.5 but for some reason R graphics don't get embedded in XML results file. Does anybody know a trick?
30 September 2013 1,625 74 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...
30 July 2024 9,824 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View