reverse transcriptase PCR

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What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem?

Describe a serious ethical dilemma you have experienced. Use the 12 questions developed by Laura Nash to offer a solution to the problem.

21 February 2021 9,133 2 View

How to perform Haldane correction on SPSS?

I was wondering if anyone knew how to perform the Haldane correction for SPSS (this is the definition of the correction -...

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Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to analyze dendritic spines from tissue (Prelimbic cortex)?

Hi there, I am trying to measure dendritic spines from mouse fixed tissue, specifically, I am looking at the prefrontal cortex. I injected the PrL with a lentivirus to knock-down my protein of...

27 January 2021 9,251 2 View

High throughput screen to identify drug induced cell death mechanism?

So I am working with a drug that we know induces cell death in MDA-MB-231 cells. In other cell lines we have been able to identify that this drug induces ferroptosis. I have also done experiment's...

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How can I carry out analysis of pulp and paper properties according to Tappi standard method in the Lab?

Properties such as relative humidity (T 402sp-03), brightness and yellowness (T-452 om-02), whiteness (T-560 pm-96), opacity and absorption coefficient (T-425 om-01), tensile, breaking length and...

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PROCESS V3.5.2 macro for SPSS variable name length limitations?

I just installed PROCESS V3.5 in SPSS and am receiving the follow error when I try to run a model: ERROR: Variable names should be no more than eight characters in length. I don't recall...

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What is the kinetics that Cr6 on the surface of the soil will preferably exchange with the charge on the surface of the iron oxide nanoparticle?

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Can anyone direct me to where I can find the X-Ray elastic constants for WC-Co or just WC?

I have searched all over the internet and I seem not to be arriving at its value. I need this value to perform some XRD measurements. Any help would be greatly appreciated.

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How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

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Calculating volume of DNA required from DNA concentration?

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Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

Shyn Joseph

I dont want animations, i know what is pcr and what we mean by reverse transcription! Well, i just need the protocol in steps (writing part/how to do part). And two step literally means preparing cdna n tranfering it to new tube for the next process!

@ lavanya : r yu doing microarray in your institute?

Swapnil Ranotkar

@ marva matboly its a very nice animation but i have to know about the exact protocol for reverse transcriptase.pls if u have that then give me the links.

@ marwa: thank you!

@ lavanya: could you plz inform me the cost if you are doing it for outside samples?