I am using a protocol to screen plant extracts against yeast C. neoformans. The protocol states that extracts should be resuspended in acetone to 10 mg/mL because bacteria and yeast can mostly survive acetone concentrations up to 25% wheres the same cannot be said for other solvents.
I have problems dissolving my methanol extracts in acetone eppendorf tubes. I try my best to break up the hard parts with a pipette tip and vortexing but still dont manage to always break the larger parts up. And since the hard parts make up the largest mass of the 10 mg/mL when resuspended in aceetone - surely my MIC values are then worse (higher) than they should be if all the extract dissolved in acetone.
I would just like to know if you know of a solution to this problem.
I think that most organic solvents have an effect on bacterial growth. You have to test the effect of each solvent first.
Make the stock to 1 mg/ml and you may either use DMSO.
Try to sonicate the mixture if problem persist.
Hope it would help.
First, a question; do you have dry the extract? If there are some oils (or other substances) for example, it is possible that certain types of reactions occur and your sample becomes insoluble in acetone. You can use a blank containing only methanol or only acetone to compare with the sample.
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