Chris la Grange

6 Questions 6 Answers 0 Followers

Questions related from Chris la Grange

MassLynx - Markerlynx statistics question: averaging of technical repeats?

I performed triplicate extractions (technical repeats) from the same batch of plant material. This was done for 40 plant species. Each of the triplicate extracts were analysed via LCMS. I...

03 March 2018 6,211 4 View

Bioautography/INT (p-iodonitrotetrazolium violet) dye/formazan confusion?

I have been screening plant extracts for bioactivity against the yeast Cryptococcus neoformans. I performed agar overlay bioautography on plant extracts separated on glass-backed TLC plates. I...

02 February 2018 1,452 1 View

Should bacterial cultures be grown in triplicate before assays?

I am testing phosphate stress on a rhizobia species. I will then be doing enzyme assays on the cultures. Is it fine to grow one culture and then repeat the assay in triplicate. Or is it...

11 November 2017 9,449 5 View

I need advice on enriching a methanolic plant extract through selective extraction to improve MIC's against Candida albicans?

I have approximately 150 grams of dried plant material (Pelargonium). I have already determined the methanol extract to have significant MIC values against C. albicans. I would like some advice...

12 December 2016 8,868 8 View

Slow growth of yeast Cryptococcus neoformans on MIC assay plates?

I have been screening medicinal plant extracts against C. neoformans on 96 well plates (MIC assays) for 6 months. Lately I have been having trouble with slow growing yeast. I add INT dye to...

10 October 2016 8,590 1 View

Resuspending plant methanol extracts in acetone for MIC assays against yeast

I am using a protocol to screen plant extracts against yeast C. neoformans. The protocol states that extracts should be resuspended in acetone to 10 mg/mL because bacteria and yeast can mostly...

07 July 2016 1,887 4 View