Research partners for a PRIMA proposal?

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Jasim Ahmed

Check if I am suitable for it? I may participate.

Hebib AGGAD

Dear Ahmed

Thanks for your response. The deadline is very close so we haven't enough time to finish it for submission. But we can be partner with you in a project related to animal and diseases. We are ready to discuss project with common interest.

Best regards

Sabah Mounir

Dear all,

is it only limited to European countries?

regards

Non southern Mediterranean countries also. I will send all needed information shortly.

Dear Sabah

Link for PRIMA projects:

http://prima-med.org/about-us/prima-in-brief/

Good Luck

I think countries are needed at least....

Best Regards

Dear Habib,

Thanks so much for your rapid reply.

Best regards,

Sabah

Mohamad Mazen Hamoud-Agha

Dear Madam,

We may be intersted ...

https://www.unilasalle.fr/recherche/recherche-service-de-nos-ambitions/unites-de-recherche/

Thanks

Patrick Martin

Dear Colleague,

I have some interest to participate with you on your project.

May be, it's so short this year, deadline is 27th March.

So we can discuss and exchange on the subject for next call.

You can join me at; [email protected]

best Regards

Alessandra Ricelli

Dear Colleagues,

I am a researcher in CNR (National Research Council, in Rome; [email protected]). My field of research deals with mycotoxin prevention (in particular ochratoxin A, aflatoxin B1 and patulin) in different kinds of food commodities (mainly dry and fresh fruits, cereals). We can discuss and exchange on the subject for next call.

best regards