May be you would like to check the method in our paper:

Lyubenova L, Götz C, Golan-Goldhirsh A, Schröder P (2007) Direct effect of Cd on Glutathione S-transferase and Glutathione reductase from Calystegia sepium. Int J. Phytorem, 9(6), 465-473

Dear Samrina

I do not work with plant extract. Only with areas of the brain of mice. With plants, I only evaluate the potential use in treatment for depression or anxiety.

Please find below the title of a book that may help you in your research regrding GR enzyme and other antioxidant enzymes in plants :

https://link.springer.com/protocol/10.1007%2F978-1-60761-702-0_16

Plant Stress Tolerance pp 273-280

Part of the Methods in Molecular Biology book series (MIMB, volume 639)

Spectrophotometric Assays for Antioxidant Enzymes in Plants

Authors

Authors and affiliations

Sathya Elavarthi

Bjorn Martin

Abstract:

Methods Mol Biol. 2010;639:273-81. doi: 10.1007/978-1-60761-702-0_16.

Spectrophotometric assays for antioxidant enzymes in plants.

Elavarthi S1, Martin B.

Author information

Abstract

Reactive oxygen species (ROS) are formed in biological systems as part of normal metabolism. Adverse environmental factors like drought stress result in increased levels of ROS that are detrimental to the plant (1, 2). To avoid damage caused by these excess ROS, plants have developed elaborate mechanisms to manage them at sustainable levels. Enzymes play an important role in lowering the ROS levels and helping avoid oxidative stress. Superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase play a vital role in combating oxidative stress. Measuring these enzyme activities spectrophotometrically provides researchers an easy and precise way to study and understand an important part of the defense against oxidative stress. In this chapter we provide details of the assays we used to determine the enzyme activities spectrophotometrically. Antioxidant enzyme responses to moderate water-deficit stress were studied. All enzyme assays were conducted using wheat leaf tissue.

PMID:

 20387052

 DOI:

 10.1007/978-1-60761-702-0_16

Thanks Ms sahar

I saw this book but problem is this ,their is different chemicals amount mentioned in Material portion and Method area,Which one is right please tell me .also how much amount are used in assay it is also not mention. Please guide

Hi,

Check the work of Carlberg and Mannervik, 1985

https://www.ncbi.nlm.nih.gov/pubmed/3003504

I hope this will help you

Heloo

I am not access above  website. please share pdf file with me.please share Smith etal1988 protocol to me 

Find the attached pdf