Are there any differences between setting automatic or manual number of measurements in DLS?

When doing measurements in DLS (either size or zeta potential), I have the option to input an automatic number of measurements or to set it manually. My question is: is there a drawback in...

08 February 2021 9,423 6 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Is there same kind of limit when annealing two DNA products?

Hi there I'm planning on annealing two PCR products to perform some mutagenesis in a plasmid. I was recommended to try and keep it to 600pb each, for example, but for one of the plasmids there...

15 November 2020 8,656 3 View

Does anyone know how to quantify C-S-H in cementitious materials using XRD?

10 August 2020 4,207 11 View

What would be the best marine bioindicator to perform micronucleus counting?

I intend to evaluate mutagenesis and genotoxicity of marine pollutants and I think that freshwater plants and animals may not be the best bioindicators.

27 July 2020 2,818 1 View

Anyone with experience in virome sequencing using Oxford nanopore?

I am interested in sequencing the phages present in environmental samples (bioreators, seawater, soil...) using long reads from Oxford Nanopore sequencing. I would like to know if anyone has been...

06 April 2020 2,972 1 View

What is the best method to measure diferences in protein-DNA binding?

More specifically, if my protein (transcription factor: TF) is known, the binding site is also known. I have a couple of TF mutants and want to quantitatively measure the differences in binding...

31 March 2020 4,828 2 View

How to prevent non-normative behaviors developed from the consumption of online pornography by adolescents and young people?

Our investigation group is currently working on a research about pornography consumption in adolescents and young people. We'd like to read your comments and any information you consider...

22 March 2020 6,127 3 View

How can be measured the fuggitive dust emissions when using a dome structure?

Hello, I am studying the reduction potential of fugitive dust emissions when using a dome structure technology for the storage of raw materials in a Sinter-making and Coking industries. I was...

18 March 2020 925 1 View

Has anyone worked with in-fusion cloning?

Hello everyone We've been struggling with our project due to low efficiency rates + check the insert is OK and not in reverse + PolTaq did not introduce a new mutation... and it has come to our...

23 January 2020 6,072 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View