I have repeated my assay to assess for synergy multiple times, and after plotting the median effect plot, I have calculated the m values and Dm values for both single agents and combination. From this I have calculated the CI values and have plotted the combination index plot. However, each repeat gets slightly different m and Dm values which affects the combination index plots. How can I take my repeats into consideration? I would like to get the mean result from my repeats. I have thought about taking the mean m and Dm values and generating a combination index plot from the mean values, will this be ok?
Ideally you would want to build run-to-run variation into your uncertainty estimates.
You can probably do that directly by introducing a random effect for run into your statistical analysis, or possibly by resamplng CI values over several runs (i.e. bootstrapped estimate of uncertainty)/
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