Hi
Is there any way to remove/separate all the membrane lipid components from the HEK cell lysate?
Ultracentrifugation (e.g. 100,000 x g for an hour) can be used to pellet the plasma membranes and all membrane organelles, leaving only the cytoplasmic proteins in the supernatant.
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
A molecule shows the -11.6 kcal/mol and second one -8.0 kcal/mol so why molecule (A) revealed higher binding score. Please explain major factor involve in it.
21 July 2024 954 5 View
Dear All, I want to cut PDMS sheet with thickness of 0.8 mm to rectangular shape with Graphtec cutter plotter CE6000-40. What condition do you offer? Do you offer any kind of special speed or force?
25 June 2024 8,111 1 View
How can we prove the strong electronic interaction between the catalyst and carbon cloth support?
19 April 2024 9,216 0 View
Hlo Researchers Any one can tell me how to isolate or estimate bacteriocins from lactobacillus spp producing strains from MRS media ? 1) At which stage it will be more suitable for sample...
28 March 2024 1,498 0 View
I want to use the TriFECTa RNAi kit for transfection, but I dont know how much RNA and control transfection should I use.
12 March 2024 1,515 2 View
Spirituality means something that actually you are, but without senses and body. But without senses and boy, what is left? and what is reach of present scientific approach.
20 February 2024 3,601 3 View
The variables under study are of mixed orders of integration i.e., I(0) and I(1). I want to estimate the long-run relationship between variables therefore it is recommended to apply pooled mean...
17 February 2024 6,566 3 View
If a compound has a lot of oxygen moities in ligand, so the docking score will be increase? If it will increase then what is the reason behind it?
16 February 2024 2,160 4 View
In the attached image, D1 dummies are for interaction term of public debt for each state, and D2 is a single dummy for structural break of Fiscal Responsibility Legislation implementation year...
27 January 2024 8,387 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View