As there are large variety of proteins, there is no universal sample rehydration buffer. But still you can use basic buffer having following composition as rehydration buffer is used to denature and solubilize protein samples

• 8 M urea or 9 M urea
• detergent concentration 0.5–4%
• DTT or DTE at 20 mM to 100 mM .
• High ampholyte concentration
• Final concentration must be optimized based on the solubility of your proteins.
• Regards

As there are large variety of proteins, there is no universal sample rehydration buffer. But still you can use basic buffer having following composition as rehydration buffer is used to denature and solubilize protein samples

• 8 M urea or 9 M urea
• detergent concentration 0.5–4%
• DTT or DTE at 20 mM to 100 mM .
• High ampholyte concentration
• Final concentration must be optimized based on the solubility of your proteins.
• Regards

Dear Sanjay,

getting your desired results depends on the composition of the buffer that is used to treat your proteins. If you have your proteins in SDS sample buffer, for example, I would not recomend to use this sample for IEF without a prior purification step (maybe precipitation) to ged rid of the SDS.

I will be able to give you useable answer if you can provide us with more detailed information on how your protein is treated or in what kind of buffer it is actually stored in.

Best, Murat

Thanks Abhishek and Murat,

Portein is in the phosphate buffer and its PI is ~ 6.9