Regarding the staining of lipid bodies in microalgae, what is the effect on lipid staining while storing under at -40C?

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Dola Bhattacharjee

Hi Abhishek

Lipid bodies in algal cells can be stained with 1μg/ml Nile Red (Greenspan et al., 1985) or 1μM BODIPY-505/515 (Cooper et al., 2010) and observed under a fluorescence microscope. For reference please look into the following literature:

1. http://algalbiofuels.pbworks.com/f/Cooper_2010.pdf

2. Greenspan P, Mayer EP, Fowler SD (1985) Nile Red: A selective fluorescent stain for intracellular lipid droplets. J Cell Biol 100: 965-973.

3. http://www.ijbpr.com/admin/fckeditor/_samples/php/article/204_677-683.pdf

Best

Dola

Can also look into:

http://www.ncbi.nlm.nih.gov/pubmed/23138061

Abhishek Sahu

Thanks!! Ms Bhattacharjee