Is it okay to use dried cells (from hot air oven, 65 degree celsius for overnight) for PHA extraction. Notably, most research articles have used freeze-dried cells for extracting PHA.
can. only the release of polymer from cells can decrease.
Thanks Dr. Evgeniy..meaning the decrease in PHA yield...??
yes
You can even dry them up to 100 ° C, in my case, drying them so much helped me to extract a greater amount of PHB using chloroform.
Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)
06 August 2024 6,610 4 View
Generally, the target material is Mo, Co, Cu, Fe, Cr, etc, so on what basis should we choose this material?
31 July 2024 5,824 0 View
give suggestions
22 July 2024 5,664 2 View
I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...
19 July 2024 1,086 1 View
How does the quarter wavelength affect the efficiency and light absorption of the cells, and what considerations need to be taken into account when applying this concept to optimize the thickness...
10 July 2024 647 5 View
..
10 July 2024 3,444 4 View
The topic should relate to pharmacy ,drug, methos of drug discovery, or any other topic but related to dug or pharmaceutical field
02 July 2024 4,031 3 View
Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...
25 June 2024 1,197 0 View
Will silver coating be able to hold such a high temperature? And will there be any effect of coating thickness?
23 June 2024 8,550 0 View
1. Role of NHRC in India. 2. UDHR 3. Constitutional rights
19 June 2024 9,616 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...
01 August 2024 9,993 3 View