Deepti Yadav

7 Questions 5 Answers 0 Followers

Questions related from Deepti Yadav

Calculating PHB yield using GC/MS?

what is the way to calculate total PHB content using Internal Standard or External standard? How to prepare a calibration curve using an external standard?

17 December 2021 9,978 6 View

Regarding PHA extraction from bacterial cells?

Is it okay to use dried cells (from hot air oven, 65 degree celsius for overnight) for PHA extraction. Notably, most research articles have used freeze-dried cells for extracting PHA.

14 December 2021 2,695 4 View

GC-MS sample preparation?

During sample preparation for GC-MS, Where i followed the given protocol: 10 mg of dried biomass + 1.7 ml methanol + 2 ml chlorofoam + 0.3 ml H2SO4 ----kept in water bath (100 degrees) for 140...

05 December 2021 8,253 7 View

Calculating PHB yield using GC/MS?

what external and internal standards can be used for quantitative determination of PHB (or PHB yield) while using GC-MS??

02 December 2021 2,780 3 View

Looking for alternatives to be used in place of corn steep liquor/ powder?

Corn steep liquor/ powder has been used as an important component in various fermentation medium. Since, the product has been discontinued from various companies, I am looking for its...

10 November 2021 1,937 0 View

Can carbenicillin be substituted for ampicillin when selecting for clones which consists of plasmid with ampicillin resistance gene?

Looking to avoid getting satellite colonies on my transformation plate!

26 April 2021 4,616 2 View

Can i reuse Ni-NTA column for the same protein? Also, washing with 0.5 M NaOH didn't seem to work (as per the manual))?

Can i simply wash with MilliQ to wash off the imidazole from the column in order to run the second set of purification of the same protein??

15 December 2019 5,668 10 View