Hi, researchers. I want to treat spectrum images (EELS) using Python ?

Hi, researchers, I want to use Python to treat spectrum image (EELS), such as denoising. But I do not know how to extract EEL spectrum form SI, like the ROI in DM. Is there any Python packages to...

06 July 2018 3,142 2 View

Refining S_L & D_L coefficients using FullProf?

Hi, researchers, when I refine the S_L & D_L coefficients using FullProf, there seems errors happening as Fig. I attached shows. However, when I fixed the Asyms and background parameters, the...

10 November 2017 5,957 0 View

How to change the "B_aniso" to the "B_iso" in EdPCR?

I use EdPCR to make a PCR file to refine the Y2O3's structure using FullProf. the way I choose is Loading a CIF file to make the PCR file, and in the PCR, it is the B_aniso parameter that is...

06 July 2017 508 0 View

What is the Oxygen stacking in the layered oxides?

As we know, there are several structures for layered oxides, such as O1, O3, P1, P3, O2. The way of oxygens stacking in the O2 structure confused me, as it seems that there are two conditions:...

06 July 2017 1,781 2 View

About the FullProf.ins file in FullProf software?

Hi, researchers, In the 50th page of FullProf manual, it mentioned that the physical meaning of some parameters is discussed in the FullProf.ins file, as the attached Fig. shows. However, I do not...

06 July 2017 4,626 2 View

How can I get the structure factor expression ?

Hi, Researchers, Recently, I am learning a tutorial of the Rietveld refinement online. It says that the structure factor of P42/mnm TiO2 can be expressed as the plot1 shows, the A factor. I want...

04 May 2017 4,739 8 View

What the definition of "Channelling" in EELS ?

Recently, I read some papers about EELS/STEM. In these articles, the term of "channelling" occurred repeatedly, such as "channelling effect", " channelling contrast" and so on. For contrast, I...

02 March 2017 4,907 1 View

Are there anyone research on expitaxial Li2MnO3 for thin film batteries ?

02 March 2017 5,515 2 View

Can anyone help me for converting file format in QSTEM ?

I am trying to convert some cif files to .xyz file format for TEM simulation with QSTEM. All cif files obtained via Findit2009 and I followed the guideline:...

02 March 2017 9,932 11 View

Is there any database about the conductivity of transition metal oxides or metal nitrides?

03 April 2014 6,084 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View