Cancer research using bioinformatics tools - any suggestions?
Hi All, I am using Ficoll Plaque based isolation of mesenchymal stem cells from umbilical cord blood. I see reduced cell numbers after Day 6. What would be the key factors to account the quality...
19 July 2023 9,954 2 View
I am trying to generate a gene knockdown in THP1 cells. I could successfully do the transduction however , i am not able to generate the single cell clones of THP1. I prepared the serial diluation...
30 May 2023 8,146 0 View
kindly try to give answer in step by step form or short video.
22 July 2022 6,390 1 View
Need to know the procedure how to use the genetic algorithm option which is already available in CST and extraction of the results.
11 July 2022 1,212 0 View
Dear all, I am facing inconsistency between my DOS and band structures calculated from VASP. I have used the same algorithm IBRION = -1 and ISIF = 0, but ISMEAR = -5 for DOS and 0 for bands. From...
21 December 2021 8,064 7 View
Dear VASP users, Suppose I add extra electrons in a system using NELECT in VASP and running scf calculations, then in the middle i am getting an error BRMIX: very serious problems the old and...
11 April 2021 2,671 1 View
Hello Everyone! I have prepared liposomal formulation using Rotary Evaporator method followed by dialyzed the liposomal Suspension in Phosphate buffer to remove the un-encapsulated drug. After...
25 January 2021 7,979 4 View
It might be a very basic question, but can somebody please put some light that what is the advantage of using Density Functional Theory (DFT) over tight-binding approximation or vice-...
31 August 2020 5,139 3 View
Dear all, Can somebody please put some light on how the position of band inversion, near or away the Fermi level affects the electrical transport and thermal properties in semi-metals? Thanks in...
19 August 2020 5,373 8 View
The protein (55KDa) has low contentration. It is purified via ammonium sulphate salting out and desalted with pD10 column. Will working with SDS-PAGE (10% resolving and 5% stacking), a drag is...
05 June 2019 4,385 14 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello everyone, I usually use the saphenous vein to collect blood from HIV-infected mice (for viral load, CD4/CD8, etc.). However, I would like to know what methods other researchers are using to...
27 June 2024 7,707 2 View
Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...
15 June 2024 3,208 4 View
My name is Carl GA Jacob. I am an Auxiliary Professor in the School of Nursing at the University of Ottawa. I am the author of the 2012 research titled: The Use of Experiential Learning in the...
10 June 2024 6,253 0 View
define cd
06 June 2024 9,533 5 View
I'd like to receive notifications of call for papers for any journal pertaining to my expertise (immunology, rheumatology, immuno-mediated liver involvement) from researchgate. Thanks in advance...
01 June 2024 1,352 0 View
In 2024, childhood tuberculosis will still be a significant global public health issue, especially in underdeveloped nations (LMICS). It is true that the underestimation of the disease's extent...
31 May 2024 5,221 0 View
Hi all, I'm trying to validate an ELISA against anti-drug antibodies in which I use a immunized rabbit serum as positive control. Rabbit was a NZR, immunization protocol was "standard" but I...
13 May 2024 2,828 0 View
