What could be possible reason of higher Drug Concentration of Liposomal formulation stability samples over time?

Hello Everyone! I have prepared liposomal formulation using Rotary Evaporator method followed by dialyzed the liposomal Suspension in Phosphate buffer to remove the un-encapsulated drug. After...

25 January 2021 7,752 4 View

What is the advantage of DFT over tight-binding methods or vice-versa?

It might be a very basic question, but can somebody please put some light that what is the advantage of using Density Functional Theory (DFT) over tight-binding approximation or vice-...

31 August 2020 4,937 3 View

How the position of band inversion (near or away the Fermi level) affects the electrical transport and thermal properties in semi-metals?

Dear all, Can somebody please put some light on how the position of band inversion, near or away the Fermi level affects the electrical transport and thermal properties in semi-metals? Thanks in...

19 August 2020 5,202 8 View

My protein is a recombinant protein constitutively expressed in Pichia.Why am I getting a smear/ dragging of the loaded protein?

The protein (55KDa) has low contentration. It is purified via ammonium sulphate salting out and desalted with pD10 column. Will working with SDS-PAGE (10% resolving and 5% stacking), a drag is...

05 June 2019 4,216 14 View

How to perform band structure calculations with HSE + SOC in VASP?

Can Somebody please tell me that how to perform band structure calculations with HSE + spin-orbit coupling (SOC) in VASP? In normal HSE calculations, we perform SCF claculations with WAVECAR of...

07 January 2019 1,310 6 View

What is the meaning of parity of a band?

As we know, a band has partial contribution from all the orbitals of all the atoms, then how can we determine the parity of a band from these partial contributions? What is the physics involved in...

22 February 2018 5,256 8 View

Is there any theory which explains the origin of the existence of indirect band gap for a material?

As we know, Kronig Penney model explains the existence of band gap at the same 'k', i.e. direct band gap. However, there is nothing like indirect band gap in KP model. Then, how can we explain the...

02 May 2017 7,619 3 View

What are High symmetry points in a crystal?

12 February 2017 6,848 7 View

How can we include Van der waals interactions in VASP 5.3.3?

I want to consider the van-der-waal interaction between two layers of Graphene. How can we include that? I have made a POSCAR file in which i considered the unit cell of both layers and the...

01 June 2016 5,626 11 View

How to find HOMO and LUMO levels in CV Measurements?

We performed the cv measurements using Ag/AgCl as reference electrode and Pt as working electrode. The analyte was added into Orhodichoro benzene (odcb). How can we find the homo and lumo level...

07 September 2014 5,871 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Is anyone else having a similar situation?

I'm looking for the genetic sequence for the TAR region of HIV. If I put Transactivator Response Region, and HIV, and "genetic sequence: into a browser...is it not unreasonable to expect to be...

10 February 2021 658 5 View

Do you think this is a major setback for Merck and the SARS-CoV-2/COVID-19 vaccine development?

Vaccine manufacturer Merck Discontinues Development of SARS-CoV-2/COVID-19 Vaccine Candidates. The company found that the vaccine shots offered less protection than just contracting the virus...

25 January 2021 1,660 2 View

Intranasal immunization with a DNA-vaccine - any advice about transfection reagents or adjuvants?

Hallo everyone! My lab is engaged in a project on creatin a candidate DNA-vaccine. As a spin-off of the main work we want to try intranasal immunization of mice with our experimental DNA-vaccines....

20 January 2021 8,221 2 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Can HIV drugs will be a provisional or recurring option against COVID-19?

Can HIV drugs will be a provisional or recurring option against COVID-19?

22 December 2020 7,872 2 View