Im doing Antioxidants' superoxide radical scavenging test. I only have NAD instead of NADH in my lab.Is there any ways that i can do to undergo reduction of NAD to NADH?
There are enzymatic methods using a dehydrogenase.
For example lactate + NAD pyruvate + NADH is catalyzed by lactate dehydrogenase, which is available commercially at low cost.
After the reaction, you can remove the protein by passing the reaction solution through a centrifugal ultrafiltration device. The protein stays behind and the NADH passes through. There will be some residual NAD, pyruvate, and lactate in with the NADH.
You could also use alcohol dehydrogenase: ethanol + NAD acetaldehyde + NADH
Wow. thats great. Thank you very much for the info. will read some more deeper for it. thanks :)
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