Mature Red blood cells don't have Nucleus . So,we can't culture them . Is there any technique when we can culture them in in vitro medium ? From ESC ? Or any other technique, suggestion ? I would like to give a trial .
Hi Babu
In vitro culture of red cells is well established. In fact, there are a number of books and monologues on this subject. You may want to access this paper if you can. Good luck
Siva
Nature Biotechnology 20, 467 - 472 (2002)
doi:10.1038/nbt0502-467
Thi My Anh Neildez-Nguyen
Henri Wajcman
Michael C. Marden
Morad Bensidhoum
Vincent Moncollin
Marie-Catherine Giarratana
more authors of this article
Human erythroid cells produced ex vivo at large scale differentiate into red blood cells in vivo
Thi My Anh Neildez-Nguyen1, Henri Wajcman2, Michael C. Marden3, Morad Bensidhoum4, Vincent Moncollin1, Marie-Catherine Giarratana1, Ladan Kobari1, Dominique Thierry4 & Luc Douay1,5
New sources of red blood cells (RBCs) would improve the transfusion capacity of blood centers. Our objective was to generate cells for transfusion by inducing a massive proliferation of hematopoietic stem and progenitor cells, followed by terminal erythroid differentiation. We describe here a procedure for amplifying hematopoietic stem cells (HSCs) from human cord blood (CB) by the sequential application of specific combinations of growth factors in a serum-free culture medium. The procedure allowed the ex vivo expansion of CD34+ progenitor and stem cells into a pure erythroid precursor population. When injected into nonobese diabetic, severe combined immunodeficient (NOD/SCID) mice, the erythroid cells were capable of proliferation and terminal differentiation into mature enucleated RBCs. The approach may eventually be useful in clinical transfusion applications.
Hello Dr. Muttuswamy Sivakumaran,
Thanks for the paper link. I will surf for more research article.
Sincerely,
Babu Mia
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