What is the maximum overall column length suitable for LC-MS using a 50 mm HILIC column?

Curious about your research experiences. Ideally this is for ESI-QTOF electrospray ionisation. This will differ based on type of columns and equilibration as per dimensions, flow rate and size of...

09 October 2017 5,453 6 View

Are there any literature sources on coupling of HILIC column to another separate stationary phase?

08 September 2017 8,659 6 View

What research techniques have you learned over the years allowing you to quickly finish research?

For me cite them right website for referencing got lab reports done easy with references. Learned web of science makes results so narrow and easy to find. Google scholar let me find cited articles...

08 September 2017 5,953 2 View

I want to test my HILIC column with different mobile phase %. Do i need to equilibrate 2 hrs per mp %?

the water layer would have formed using 85%. Is the same amount of water layer formed by each acetonitrile %.what I am asking is for 97% if I equilibrat for 2 hrs then can I equilibrate for 30 to...

08 September 2017 7,296 11 View

How much of a water layer is required in HILIC column to effectively bind hydrophobic compounds?

,Tried 3% water layer but equilibration time is time consuming. Analyte had a curved out looking shape such as an upside boat that continued for 2minutes to reach the baseline. steep then smooth...

08 September 2017 4,633 9 View

For my HPLC UV detection can I apply 0.1% formic acid and 10mM Ammonium acetate at pH 4 to see what effect the buffer combo can have in HILIC?

I read in Mass spec literature that buffer combinations are attempted. Are they only for gradient elution. Does this need mass spec to ionise the buffered solvent effectively. Will this cause the...

08 September 2017 617 4 View

What are some good examples of articles for moderately polar compounds separated by HILIC?

LogP

08 September 2017 720 5 View

I have an impurity from a purchased bacterial standard. What methods do you use to analyse if it is a standard besides HPLC-UV detection?

08 September 2017 7,121 2 View

What are the typical To void volume markers used for mixed mode chromatographic columns that do not retain hydrophobically/ hydrophilically?

This situation can be applied to 2D chromatography. If there is a T-piece can two To void volume markers be used to measure that volume for sample to reach the detector. Some research applies...

08 September 2017 9,947 5 View

Are there any infra red detectors for HPLC or UV+IR detector for liquid or gas or supercritical fluid chromatography?

Just wondering about their expense to make. Does liquid propulsion cause excess interference in IR?. A multifunctional HPLC that checks for impurities would be more valuable. Does the liquid...

08 September 2017 7,457 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View