Looking for a good polyclonal anti-RFP antibody with low background for mCherry detection. If you have a link to the paper you used it in so much the better!
As follow up to this question we decided to go with the Clontech anti-DsRed polyclonal antibody and have had some good success.
We have recently replaced the tip seals on our scroll pump and since it came back our scroll pump has started acting oddly where the backing line settles at about 1E-1 mbar where previously it was...
12 March 2024 6,280 2 View
is there an Excel formula to calculate depth of the mixed layer using seawater density profile?
21 February 2024 3,328 2 View
I am trying to find the works of a researcher (I think an anthropologist) who wrote on indigenous land management in California. In particular, he argued that this management was not as spatially...
10 February 2024 6,241 3 View
After Gram Staining, culturing is the second step in identifying causative agents which is time consuming and prone to errors.
03 February 2024 8,777 4 View
I'm attempting to run RDA on a SNP dataset to search for effects of isolation-by-distance (IBD). The script I have now (Meirmans, 2015: Seven common mistakes in population genetics and how to...
26 December 2023 3,226 1 View
For my Dissertation, I am looking at either soil pollution or water pollution. I am curious about heavy metal pollution and how heavy metal pollution affects local communities and ecosystems by...
26 December 2023 4,883 7 View
I am working with real-world data. I want to test the effect of outside missionaries (specifically their departure) on the number of Catholics in a jurisdiction (either countries or dioceses). My...
29 October 2023 7,446 1 View
Hi all, I am trying to extract RNA from snap frozen leaf tissue ~100-150mg. I am getting good yields >150ng/uL normally with 260/280 >2.1 and 260/280 >2 as well. This is a good sign,...
16 October 2023 4,678 3 View
The different effects of different concentrations of salt in water and how they may affect germination and growth of plant seeds. The sources of info that can offer more details on conducted...
08 September 2023 2,299 1 View
Do federal policies on climate change help reduce carbon emissions
26 August 2023 6,686 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View