Unusually Busy Raman Background?

Hello, I am observing a nearly periodic background spectrum for a wide variety of SERS substrates. I see this response (attached below) for bare Au films, Cu films, and Au nanoparticles on...

15 February 2021 9,922 5 View

What crossover operators can be used for a GA that finds suboptimal positions for a set of buildings?

I'm currently working on my undergraduate thesis where I develop a genetic algorithm that finds suboptimal 2D positions for a set of buildings. The solution representation is a vector of real...

11 January 2021 9,835 4 View

How did Rutherford and Thomson derive the equation for average deflection of a particle hitting an atom?

08 November 2020 9,046 2 View

Converting csv files with data for mapping rainfall on QGIS?

Hi, I am hoping to produce rainfall and temperature maps for a current project in QGIS. I have csv data downloaded which have been imported into QGIS. My aim is to be able to produce rainfall...

10 October 2020 4,930 5 View

How to control variables in a water hammer experiment?

I'm doing a high school experiment on how the impulse of water hammer is affected by pipe diameter, and I'm unsure of how to set up my experiment. My primary concern is safety and validity: - Is...

06 September 2020 1,252 3 View

Incorrect simulated conductance value compared to analytical solution using ANSYS Maxwell 2D?

31 May 2020 7,991 3 View

Reaction of hexamethyldisiloxane (HDMSO)+O2=SiO2+?

I'm giving a presentation on semiconductors and specifically the SiO2 insulating layer. The presentation is on kinetics so I kind of need to know the reaction. Problem is the papers I'm reading...

30 March 2020 8,643 3 View

Efficient preps for chunky human adipose tissue?

Hi all, I aim to isolate SVF from the tissue. However, we sometimes receive big chunks of tissue that is not through liposuction. I've been wondering is there a ingenious way to efficiently prep...

24 February 2020 6,104 1 View

Predicting the Age of home buyers - model based method?

All, I have a data-set which contains information on both the type of property an individual(s) purchased, along with some information on the individuals themselves. However, I am missing age...

19 February 2020 5,728 3 View

Are full-scale performance-based drama projects for adult EFL learners worthwhile?

Hello, I wonder if anyone would be kind enough to help with research for my dissertation concerning attitudes and approaches to full-scale stage or screen performance-based drama projects for...

27 January 2020 3,167 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View