Recommended cleanup procedure for glassware used with Karnovsky's fixative/glutaraldahyde/paraformaldahyde?

04 April 2016 1 8K Report

And can plastic disposable pippettes/tips be flushed with water, allowed to vent in a fume hood overnight, then disposed of with non-contaminated sharps?

Krishnat S Yadav

Yes, you can do that but instead of only water, you can use glutaraldehyde. Then rest is ok.

Badges
Science topic

More Ariel Parker's questions See All

When would using hardening vs non-hardening mounting media for fluorescent microscopy of fixed cells be appropriate?

Methanol-fixed cytospin slides I'd also like to stain with DAPI. The cells have (likely) uptaken fluorescently labeled nanoparticles.

03 April 2016 8,374 3 View

Statistical analysis on cell differential data?

I've never done this before,but have been given instructions how to do some ANOVA analysis using a program called JMP. However, that program might be unavailable to me at the moment and I was...

03 April 2016 7,177 3 View

Mounting media with DAPI for staining cytospins?

Hi, I have some ctyospins of cells exposed to fluorescent nanoparticles, I'd also like to stain the slides with DAPI to prepare for confocal fluorescent microscopy. Is there an advantage to...

03 April 2016 4,526 3 View

How to do tissue preparation for confocal microscopy?

I have mouse lungs fixed in 4% paraformaldehyde and am unsure how to prepare them for confocal microscopy. I'm looking to do a quick check if the Alexa Fluor 680-labeled nanoparticles within the...

09 October 2015 9,557 6 View

Can rat tissues (lungs, nasal cavities, brains) stored in 4% PFA, refrigerated, for a couple years be used for electron microscopy?

It' s my understanding that for TEM analysis, tissues must be fixed in glutaraldehyde (or 2% PFA and 2% GTA solution) to be embedded in resin, whereas tissues fixed in PFA is embedded in paraffin.

05 June 2015 3,913 3 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

Project/Experimental assistance ？?

Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...

05 August 2024 5,829 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal?

Any idea about 'International Research Journal of commerce , arts and science? Is it a UGC listed journal? Kindly advice

04 August 2024 7,367 3 View

Are authors sowing for scientific Journals to be reaping the benefits? We are charged for publication but we offer free peer review services, why?

As authors and academic writers, we usually prepare our manuscript using our own resources. We submit these manuscripts to scientific Journals for peer review and publication sometimes at a fee....

03 August 2024 7,304 2 View

Short Synthesis of Graphene Oxide from Natural Graphite Flakes?

I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...

01 August 2024 8,368 2 View

Does continues partial charging/discharging do any harm to the BESS performance and degradation?

For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...

01 August 2024 9,903 7 View

Is it true that science is leaving the era of mathematics and entering the era of matrix mechanics?

We assume this to be true. Science leaves the era of mathematics and enters the era of matrix mechanics and the turning point is the discovery of numerical statistical theory called Cairo...

31 July 2024 3,900 2 View