Ariel Parker

6 Questions 2 Answers 0 Followers

Questions related from Ariel Parker

Statistical analysis on cell differential data?

I've never done this before,but have been given instructions how to do some ANOVA analysis using a program called JMP. However, that program might be unavailable to me at the moment and I was...

04 April 2016 7,141 3 View

Mounting media with DAPI for staining cytospins?

Hi, I have some ctyospins of cells exposed to fluorescent nanoparticles, I'd also like to stain the slides with DAPI to prepare for confocal fluorescent microscopy. Is there an advantage to...

04 April 2016 4,424 3 View

When would using hardening vs non-hardening mounting media for fluorescent microscopy of fixed cells be appropriate?

Methanol-fixed cytospin slides I'd also like to stain with DAPI. The cells have (likely) uptaken fluorescently labeled nanoparticles.

04 April 2016 8,332 3 View

Recommended cleanup procedure for glassware used with Karnovsky's fixative/glutaraldahyde/paraformaldahyde?

And can plastic disposable pippettes/tips be flushed with water, allowed to vent in a fume hood overnight, then disposed of with non-contaminated sharps?

04 April 2016 7,754 1 View

How to do tissue preparation for confocal microscopy?

I have mouse lungs fixed in 4% paraformaldehyde and am unsure how to prepare them for confocal microscopy. I'm looking to do a quick check if the Alexa Fluor 680-labeled nanoparticles within the...

10 October 2015 9,523 6 View

Can rat tissues (lungs, nasal cavities, brains) stored in 4% PFA, refrigerated, for a couple years be used for electron microscopy?

It' s my understanding that for TEM analysis, tissues must be fixed in glutaraldehyde (or 2% PFA and 2% GTA solution) to be embedded in resin, whereas tissues fixed in PFA is embedded in paraffin.

06 June 2015 3,866 3 View