At what step should one add internal standards in tissue sample for LC-MS?

Hi everyone, I'm doing metabolomics of placental tissue in my lab. I added 1ml of 80% MeOH along with 2 isotopic internal standards namely, phenylalanine and palmitic acid to my sample after...

18 January 2021 9,520 4 View

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26 July 2020 1,511 0 View

How to connect Lithium ion cells (modelled using 2RC equivalent circuit approach in simulink) in series and parallel?

I have modelled a lithium ion cell in simulink using the 2RC equivalent circuit approach. I want to connect multiple cells in series and parallel to form a complete battery pack. In the literature...

13 June 2020 4,107 4 View

What is the COVID-19 Test Kit market size and industry forecast ?

COVID-19 Impact Analysis on COVID-19 Test Kit Market report published by Value Market Research provides a detailed market analysis comprising market size, share, value, growth and trends for the...

04 May 2020 9,241 3 View

How to calculate the leaf area from a drawn outline of a leaf on graph paper ? means is there any easy software or any other method to calculate ?

I have drawn the out line of leaves on a graph paper, and i have like 120 pages of sheets each containing 5 graph on an av. so its actually laborious to calculate it manually. I thing MATLAB is...

23 January 2020 7,544 3 View

Do we need to perform a pilot study in qualitative research? if yes, how?

If semi structured questions are designed to collect the data, do the statements require a feasibility test? If necessary, what is the process?

26 November 2019 328 17 View

Can i use stress from the predefined fields to apply confinement pressure for my SMA confined concrete model?

i want to model SMA confined concrete for that i need to apply prestress for the recovery stress gemerate as confining pressure in hoops arround the cylinder.

24 January 2019 2,804 3 View

I am looking for a simultaneous heat and mass transfer model with finite differences?

I am trying to come up with a code for simultaneous heat and mass transfer for water evaporation owing to solar flux. The incoming heat flux contributes to volumetric heat generation within a...

24 October 2018 8,964 1 View

How long can GFP plasmid DNA withstand sonication without hampering its expression?

11 April 2017 2,179 3 View

What is the probable reason behind this??

We have synthesized vanadium sulfide nanoparticle (VS2) through a simple solvothermal method. Nyquist plot( impedence spectroscopy) shows that VS2 modified glassycarbon electrode (VS2/Nf/GCE)...

27 February 2017 4,393 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

Pile-Raft connection?

Greetings, I was wondering what is the optimal way to connect the reinforcement at the top of the pile (the part that connects to the raft foundation): -The pile bars are left straight and...

16 February 2021 2,007 3 View

Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

Resume Book UEP2013 Asian Edition, dis somebodey has it?

I am looking for a copy of the resume book of UEP2013 Asian Edition (Urban Environmental Pollution 2013 Asian Edition) held in Beijing. If somebody has it, please I would like to obtain an...

11 February 2021 2,247 2 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

After transformation of Dh5alpha competent cells I found a cloudy white ppt before plating. Can anyone explain this?

After transformation of Dh5alpha cells by heat shock I grew them at 37 in 200microlitre Terrific Broth (because I had it at hand) and then before plating I found cloudy white ppt in the cell...

03 February 2021 7,867 3 View

Has someone used TTC agar overlay method and can help me?

I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...

22 January 2021 585 10 View

I am trying the synthesis of graphene oxide by using modified hummer's method and the color of my product was white silver so is it graphene oxide?

i have tried modified hummer's method for the synthesis of graphene oxide by adding Graphite powder and NaNO3 in H2SO4 . In this mix KMnO4 was added and diluted with water . this mix was heated...

13 January 2021 9,423 4 View