Real-time primers for Bacteria?

01 January 2014 1 7K Report

I'm planning on doing molecular screening for bacteria (Domain Bacteria, no subclasses) in soil samples with real time PCR. Can anyone suggest what primer I can use for the PCR?

Paul V Flanagan

you could try primers from Gerard Muyzer (1994) P1 and P2. These target the V3 region of bacterial 16S. here is a link:-

http://aem.asm.org/content/59/3/695.full.pdf

Might be a useful starting point for you.

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