RCE-SDS of mAb: What is the peak migrating in between HC and HC-LC thioether?

17 March 2021 0 4K Report

I've been looking through literature of reduced CE-SDS of monoclonal antibodies and I frequently see this 'post-HC' peak. I don't know if it is specific to a particular IgG, but I see it frequently and no papers seem to describe what it is.
The peak migrates slightly slower than the HC, it doesn't match the HC migration when deglycosylated, and it's faster than the HC-LC thioether.
A different paper by Janssen from 2019 (10.1080/19420862.2019.1646554) points it out, but fails to identify it.
I'm curious if anyone knows what this peak is? Thoughts?

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