Rationale behind selection of 3T3-L1 adipocytes & C2C12 Skeletal muscle cell lines?

Balaji Hari @Balaji-Hari

07 December 2022 0 3K Report

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On 27 May 2024, accidentally I removed 25 papers from my list of publications on Rsearch Gate. How to restore deleted papers?

28 May 2024 3,927 1 View

Explain the geological cycle of formation of different types of rocks .what are the physical ,chemical,and biological phenomenon involved in forming?

need answer in a tleast 20 lines

21 April 2024 5,489 0 View

I treated CNTs in reduced Nickel chloride solution using sodium boro hydride. After centrifuing i got mixture of CNT and nickel. how to seperate CNT?

I treated CNTs in reduced Nickel chloride solution using sodium boro hydride. After centrifuing i got mixture of CNT and nickel. how to seperate CNT? i have done this for decorating CNTs wit...

20 April 2024 6,574 1 View

How to obtain slope distribution of an AFM scan using Nanoscope?

I have AFM scans of a uniformly textured surface of 16x16um size. I would like to obtain the slope distribution of the surface using Nanoscope, as I plan on trying to relate this parameter to the...

10 April 2024 5,024 2 View

Can anyone suggest a free citation manager alternative to Mendeley?

Over the last 10 years, I have been using the Mendeley citation manager. Due to their new policy, it is not working as smoothly with MS Word. Therefore, I am looking for a good alternative.

10 January 2024 3,460 5 View

Is the guide RNA (gRNA) designed for gene knockout has possibility of binding to messenger RNA (mRNA) of the target gene?

In CRISPR/Cas, we design guide to make double strand break in a specific site of the genomic DNA. I am curious to know, whether the gRNA-Cas9 complex has any possibility to bind to the messenger...

25 December 2023 630 1 View

How to create titianium metal nanoparticles from titanium tetrachloride ?

using solution based method

17 November 2023 8,243 1 View

Any research people wil help me in docking studies? I have chemistry work???

Iam Dr M HARIKRISHNA doing research in organic chemistry, I need help for docking studies?

03 November 2023 9,336 3 View

I want to measure the work function of fused silica. Is it possible to measure the work function using UPS? Does anyone have experience?

I want to measure the work function of fused silica. Is it possible to measure the work function using UPS? I have seen several papers using UPS, but did not get the concept behind that. Does...

02 November 2023 3,779 4 View

how to find out volume of chamber 2, using the equation state of an ideal gas by isothermal expansion of a known volume of a gas from chamber1 to 2?

how can we find out the volume of chamber 2, using the equation state of an ideal gas by isothermal expansion of a known volume of a gas from chamber 1 to chamber 2

01 November 2023 7,811 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View