Rapid Equilibrium Dialysis (RED) to measure plasma protein binding of small organic compounds:
- has anyone done this for desferioxamine B (DFOB) and what were your results?
- I'm using the ThermoFisher RED device with 48 inserts
- can one wash and reuse the inserts with reproducible results? they are cellulose membranes with 8 kD cut-off, I figure with buffer rinse and ethanol wash they should be good to use multiple times?!?
Thanks in advance