I would like to analyze the Raman spectra for my Manganese oxide nanoparticles. However, the spectra shows nothing. Is there any method to prepare the samples for Raman spectroscopy? Or if there is any other issue, please let me know.
Thanks
Dear Vishnu Revuri
Generally, the sample preparation for Raman spectroscopy usual do not require a special protocol. This is one of the coolest features of Raman spectroscopy. With regard to your case, I can recommend increasing the concentration of particles in the laser beam area. But I hope that playing with experimental parameters can give a more significant result. I believe that you use rigorous optical alignment Raman microscopy. Firstly you should be sure that laser power is low enough and you not burning you nanoparticle. Secondly, try to increase accumulation time by several times and maybe changing wavelength of excitation laser.
Dear Vishnu Revuri
Generally, the sample preparation for Raman spectroscopy usual do not require a special protocol. This is one of the coolest features of Raman spectroscopy. With regard to your case, I can recommend increasing the concentration of particles in the laser beam area. But I hope that playing with experimental parameters can give a more significant result. I believe that you use rigorous optical alignment Raman microscopy. Firstly you should be sure that laser power is low enough and you not burning you nanoparticle. Secondly, try to increase accumulation time by several times and maybe changing wavelength of excitation laser.
Dear Vishnu,
Just to add to Alexander's comments above:
1) If low power isn't working, do try higher laser power (with longer exposure time and several accumulations). Mg oxides should be pretty stable, and you can always check for sample damage by taking a spectra, looking at the signal then repeating the spectra in exactly the same location - if the spectral intensity isn't changing then there's no damage to your sample. Once you _have_ a signal you can scale it back.
2) to limit scatter of the beam, try with red lasers rather than green (as long as Mg oxides are responsive to red laser frequencies)
3) If you're working with a high magnification objective (e.g. 50x) try a lower magnification one to begin with (10-20x?) - particularly if your sample is dilute.
4) If you have the option of altering the hole-size (confocal system) then it's worth experimenting with this, particularly if your particles are very small nano dimensions.
I suspect you already have quite a lot of Raman experience so you may have tried these things already.
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