Hi, I'm currently working on my steroid receptor project. I have different receptors stained in tissue samples and currently using QuPath programme to analyze the scanned images. My question is, if I want to compare the intensity of a specific staining (both nuclear and cytoplasmic) compared to controls (strong, moderate, weak) how can I do that with QuPath? Is there any documented method? I appreciate all the answers!
I don't have experience with QuPath, but if you are still looking for a method to quantify your IHC data, you might also want to have a look at ImageJ. It's also free and open source and there's a lot of documentation online.
For your issue, see: https://www.unige.ch/medecine/bioimaging/files/1914/1208/6000/Quantification.pdf
Best,
Frank
There's a forum just for QuPath at https://forum.image.sc/tags/qupath
(Or, if you use the imagej tag, also for ImageJ)
Hi Ana,
I have right now kind of the same task! So, I'm wondering how finally you measured your stainings and if you have some suggestions about it based on your recent experience.
Best,
Pablo
P.S.: Thanks Peter and Frank for your answers, I'll check those links out! :)
- Badges
- Science method
- Similar topics
- Biological Science
- Histology
- Staining