Dear colleagues,
Does anyone have experience with fixing and permeabilizing cells to perform immunofluorescence of quinacrine? I have successfully observed the green fluorescence in live cell conditions. Nevertheless when fixating with 3% PFA and permeabilizing with Saponin, the signal is lost. I have found some papers where quinacrine signal is shown in fixed cells along with LAMP1. Sadly, the details of the fixation/permeabilization method are not clear enough.
I would appreciate any help.
Thanks in advance,
Felipe