This blog post gives the general background and a link to the original publication
http://core-genomics.blogspot.com/2012/04/how-do-spri-beads-work.html
This blog post gives the general background and a link to the original publication
http://core-genomics.blogspot.com/2012/04/how-do-spri-beads-work.html
So they serve as a precipitation point but the DNA needs to be precipitated by sth. Usually one uses high salt and PEG precipitation (similar to alcohol precipitation from the work flow, the DNA is crowded out of the solution).
The Ampure XP beads are good for standard applications. However, if you want to play around with volumes and extracted DNA length, you might want to prepare your own buffer for the beads. It is also much cheaper and described here:
http://www.openwetware.org/wiki/SPRI_bead_mix
The beads have a high binding capacity (7ug/ul beads). I generally now use a few beads and the amount of precipitation buffer that is appropriate for the DNA length I want to precipitate on top of the reaction. That makes it much cheaper again.
The beads also do not inhibit most reactions (they do inhibit PCR!). So the beads can be left in the tube during the reaction and the resulting nucleic acids can be precipitated onto the beads by addition of the buffer (PEG and NaCl). This was called the with bead method and there are some protocols out there to do that.
https://blog.genohub.com/2014/05/07/peg-size-selection-and-precipitation-of-dna-libraries-how-ampure-or-spriselect-works/
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology