Stephan Kirchmaier

2 Questions 4 Answers 0 Followers

Questions related from Stephan Kirchmaier

Reverse transcriptase with 5'3' exo activity

Hi! I am looking for a reverse transcriptase that has no strand displacement but rather 5' to 3' exonuclease activity. The closest thing I found is Bst polymerase from NEB but that is only a weak...

25 July 2017 6,643 2 View

Silencing of Cas9 in mammalian cells?

I've heard from several independent people that their Cas9 cell lines that constitutively express Cas9 is being silenced. I've heard it for neuronal stem cells as well as for HEK293 cells. I also...

18 July 2017 3,989 2 View