I am recording sEPSC(holding at -70 mV) and sIPSC(holding at 0) these days. My internal solution is Cs-based low Cl solution. But EPSC still appears when I was recording sIPSC which is obviously incorrect. My collegue is doing the same experiment and using the same solution as mine but recording in his rig doesn't have such issue. Does anyone have any idea about this? I don't know which part could be blamed. I am sure the cells are good. I would be appreciate for any suggestion. Thanks!
Dear Jiachen,
I recommend that you use pharmacological blockers to isolate your currents of interest (CNQX, APV, picrotoxin). It has the advantage that you can also check whether or not your recorded currents derive from specific ion channels, and it may eliminate your EPSCs.
Good luck!
Dear Jiachen Fan ,
Do you or your colleague apply any liquid junction potential correction?
Are you sure your system is holding the cell at the desired potential? I ask this because idk your hardware, but if you're using Axopatch 200B, sometimes people would just forget to switch on the toggle for external command.
Which potential can you actually see no EPSCs?
The more details you provide, the better for us to help out!
Andre