30 May 2025 1 8K Report

El parvovirus Canino

More Amir Aldair Vega's questions See All
"Idrisi Selva" tutorial Books for pridiction with driving factors in modeling?

Some educational books with practical exercises of the "Idrisi Selva" software, which have step-by-step visual training for predicting the change of land use/land cover and including the use of...

28 July 2024 4,168 0 View

Hello, does anyone know how I can get coordinates of two specific points on a moving body in Abaqus when applying a vdload subroutine?

Hello I am trying to simulate repulsive force of two permanent magnets in Abaqus. I have implemented Force equations into a vdload subroutine. sensitive parameters are the center to center...

15 July 2024 3,491 0 View

To what extent should "reading comprehension" be analyzed in an A.R. based on phonological awareness instruction to EFL students using a webtool?

Dear Colleagues and academy, I am conducting an action research study to understand the impact of phonological awareness activities using Quizlet and Boom on the reading comprehension skills of...

02 July 2024 8,480 0 View

Can anyone recommend an antibody development course?

I would like to find a short course focused on the development of monoclonal antibodies covering the entire process, from the selection of antigen and carrier, to the purification of the final...

27 June 2024 5,618 1 View

Is it possible to use Overhangs with large different Tm with NEB Builder HiFi DNA assembly?

Dear cloning community, Is it possible to use Overhangs with large Tm difference with NEB Builder HiFi DNA assembly? 5´ 26 bp Tm 57ºC 3´ 24 bp Tm 72ºC Unfortunately, extending the 5´ doesn't...

17 June 2024 8,552 2 View

How to install a shiny app?

For example, I want to install the app "01_hello" as an independent app (like many other apps of windows). Please find the attached screen. But after playing the app, I run into error.

22 May 2024 955 2 View

Seeking Insights on Real-Time PCR Tests with Cobas Z480: Quantitative and Qualitative Analysis?

Hello everyone, I’m delving into the functionalities of the Cobas Z480 analyzer and I’m eager to learn not only its basic operating principles but also about the different real-time PCR tests,...

20 May 2024 7,861 0 View

JCR, Scopus journals, urban planning, analysis of urban facilities and opportunities, transportation and land use ?

Please name some prestigious JCR and Scopus journals in the fields of urban planning, sustainable development, analysis of urban facilities and opportunities, transportation and land use with high...

11 May 2024 4,789 5 View

Journals by sending to multiple reviewers to give authors more opportunities to correct their manuscripts and gain acceptance?

Is it true that some journals, in case of rejection of an article by some reviewers, send it to several other reviewers so that if there is innovation in the article, justice is done and good...

11 May 2024 401 0 View

Artificial intelligences for literature review?

Which artificial intelligences can be useful in finding scientific papers related to a topic to prepare a literature review of the research (methodology, case study location, variables used)?...

10 May 2024 9,478 3 View

Similar questions and discussions
I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 1,170 4 View

Is there a comprehensive database for Virus Real-Time Primers and Probes ?

Dear All I am looking for a database for tested Real Time PCR primers and probe sequences for the following viruses for diagnostic purposes. Does the USAID PREDICT project or the Global Virome...

01 February 2021 9,210 1 View

What is the relation between bootstrap and homoplasy?

I am working with the VP2 protein DNA of carnivore parvoviruses and sequences are only of 1300bp. I obtained the following phylogenetic tree (Maximum likelihood, 1000 replicates) and the reviewer...

14 February 2019 8,713 0 View

Porcine vascular endothelial cell isolation - from the aorta?

Hello, Are there any protocols for the isolation and purification of porcine vascular endothelial cells (from the aorta)? I would like to have my study from the primary culture instead of...

09 October 2017 9,600 3 View

Hi, I'm unable to sequence a DNA fragment with a polyA region ~13bp. Any advice on if additional Mg2+ may help & suggestions for a gradient protocol?

I am sequencing overlapping regions of the VP2 gene of Parvovirus. We get a good reverse sequence but chromatograms following the polyA region in the forward sequence have very low and mixed...

23 July 2016 9,307 15 View

Hi, I'm unable to sequence a DNA fragment with a polyA region ~13bp. Any advice on if additional Mg2+ may help & suggestions for a gradient protocol?

I am sequencing overlapping regions of the VP2 gene of Parvovirus. We get a good reverse sequence but chromatograms following the polyA region in the forward sequence have very low and mixed...

06 July 2016 8,595 16 View

Transmission of HSV by allogeneic serum eye drops - is it possible?

Hello! We produce allogeneic serum eye drops from (SED) whole blood donation and test them for HIV-1/2, HCV, HBV, HEV, Parvovirus B19, Syphilis, CMV and HSV. We look for antibodies and for the...

28 June 2016 3,992 0 View

How could I isolate a specific virus from a multivalent vaccine?

I want to isolate canine distemper virus in cell culture (Vero) from a multivalent vaccine that also has adenovirus, parvovirus, parainfluenza (all attenuated) and Leptospira (inactivated). Is...

15 June 2015 573 3 View

What is the best protocol for PCR purification of Canine Parvovirus extracted from spleen of small mammals?

I have used dreamtaq and amplitaq with both plain and nested PCR but it does not seem to work really well, The image is really smeared with a lot of non specific binding when extracted on 1.5%...

13 May 2015 2,709 3 View

Do you know if duodenal papillitis could be an unusual complication of Human Parvovirus B19 Infection?

I recently observed a young immunocompetent girl with obstructive jaundice related to  duodenal papillitis and detection of Parvovirus B 19 IgM

12 April 2015 5,205 1 View

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