When we carry out quantitative analysis of alizarin red to detect calcium deposit in monolayer cell culture, then can we conclude that the concentration of calcium deposit is equal to the molar concentration of alizarin red obtained at 405nm
I wanted to find the calcium deposition quantity in monolayer culture so i performed Alizarin Red S staining quantification kit. I want to specify in result that the calculation of ARS concentration in the sample suggest the cactual concentration of calcium deposit in monolayer culture