good morning every body , i isolated DNA from tissue's plant. how to check the quality of DNA . thanks a lot
Please check the following researchgate link.
https://www.researchgate.net/post/Which_is_the_best_method_to_determine_DNA_quality_and_concentration
Please have a look at this useful researchgate link also.
https://www.researchgate.net/post/How_can_I_check_plasmid_quality
Have a look at this useful link also.
https://www.researchgate.net/post/Running_a_genomic_DNA_quality_check_on_a_gel-can_anyone_help/1
Please check below given link also.
https://www.academia.edu/706715/TOTAL_GENOMIC_DNA_EXTRACTION_QUALITY_CHECK_AND_QUANTITATION
To evaluate DNA purity, measure absorbance at A260 and A280. Good-quality DNA will have an A260/A280 ratio of 1.7–2.0. You can use a traditional spectrotometer or a Nanodrop to measure.
-----------------------------------------------------------------------------------------------------------------------------------
See attached document (yellow highlights) for more info:
"The A260/280 ratio is generally used to determine protein contamination of a nucleic acid sample. The aromatic proteins have a strong UV absorbance at 280 nm. For pure RNA and DNA, A260/280 ratios should be somewhere around 2.1 and 1.8, respectively. A lower ratio indicates the sample is protein contaminated. The presence of protein contamination may have an effect on downstream applications that use the nucleic acid samples."
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology