Q-Dots or other high yield fluorophore that exite at ~254nm and emit at ~300nm?

More Yoram Gerchman's questions See All

What is the biological importance of alkaline phosphatase in milk?

Greetings all As you might know raw milk has an alkaline phosphatase activity, and indeed this activity is used for pasteurization quality control. The question I am wondering about is, what is...

05 June 2019 4,596 1 View

Extraction of Heme A, Heme B and Heme O?

Greetings I am looking for a trusted protocol for extraction of Heme A, Heme B and Heme O, to be used as standards in HPLC analysis. Alternatively if you know of a commercial source also...

04 May 2019 5,605 2 View

Plasmid for constitutive expression of gfp in Bacillus subtilis?

Greetings Does anyone have , and is willing to sent me, a plasmid suitable for constitutive expression of gfp in Bacillus subtilis? Many thanks Yoram

03 April 2019 1,628 1 View

Illuminating a sample with 2 wavelength simulteneously?

Greetings I am looking for an optical setup that will enable me to illuminate a single sample with two different wavelength in the UV range simultaneously. I though about placing 2 monochromators...

10 November 2018 1,686 0 View

What are the carbon 'species' in traeted wastewater? A good model compound for such?

Greetings all As wastewater get biologically treated carbon is removed, but obviously not all carbon species are removed, since some are more easily consumed by the bacteria doing the treatment....

10 November 2018 6,388 2 View

Origin of 220nm absorbance peak of free amino acids?

Greetings I am trying to find out what is the origin of the 220nm absorbance peak of free amino acids? It is usually attributed to peptide bond but I find it also in single aa... Yoram

05 June 2018 6,797 3 View

Why do particles concentrated in water droplet edge?

Greeting. I have noticed a pattern and wonder what is the reason. When ever I have suspension of particles in water droplet, be it bacteria or abiotic they seem to concentrate in the edge of the...

04 May 2018 10,187 1 View

Can I connect HP1047A RI detector to Agilent 1200 HPLC system?

Greetings We need to buy an RI detector for our Agilent 1200 HPLC system (connected to a computer running ChemStation). Given the high price of new detector I thought about buying a used one....

09 October 2017 7,320 10 View

Fluorescent dye that bind to dsRNA but not ssRNA?

Greetings all. We are looking into following the kinetics of short dsRNA melting and about using a dye that will bind differently to dsRNA and ssRNA. Any idea for such a dye? Or to another simple...

06 July 2017 6,823 1 View

Quantifying UV irradiation of different lamps by UV sensor?

Greetings I am trying to quantify UV irradiation form different UV lamps buy using UV sensor. The problem is the response curve of the sensor is different for different wavelength (as it is often...

05 June 2017 7,507 4 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

What are possible strategies can be used to analyze data under sequential explanatory mixed method approach?

Better ways to analyze the qualitative and quantitative data in a sequential explanatory mixed method approaches

04 August 2024 2,703 6 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How to calculate the molar proportions of the oxides from the XRF analyses?

02 August 2024 2,565 2 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Ofir Tal

Sounds like Tyrosines abs and emission. Although I'm not sure regarding the q.y..

Fabrizio Messina

Tyrosine QY is very low. PTP is indeed a good suggestion (you can solve it in methanol or ethanol in reasonable concentration). If 350 nm emission still works, another alternative is 2,5-Diphenyloxazole..