Pseudogene EDNRA in gene expression anlysis

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How to determine Forster radius of a FRET pair?

Dear all, I'm searching for methods to calculate Forster Radii of FRET pairs, ideally for water-soluble and membrane dyes. Although I am relatively familiar with working with microscopy FRET, I am...

07 December 2020 3,051 3 View

How do I measure the fluorescence intensity over time for moving objects?

Hi all, I need to measure the fluorescence intensity for a given object (on two different channels) over time. The object moves (drifts) over time, so its position isn't fixed throughout the...

20 April 2020 3,346 3 View

How can I make input file for rehh package in R with scaffolds?

I am trying to look for positive selection in the genome of a non-model specie, therefore I do not have chromosomes structure, I just have scaffolds and positions of SNPs in a VCF file. I used...

11 March 2020 3,700 3 View

Can supported lipid bilayers be formed with cationic lipids?

Hello there, I am trying to coat plastic beads (spherotech beads) with cationic lipid bilayers. There are several protocols for neutral and negatively-charged bilayer formation on polystyrene...

24 July 2019 8,461 7 View

Does anyone have Id x Vgs and Id x Vd measurements of any organic field effect transistor?

I'm only finding plotted graphs. But I needed all the measurement values (Id Vgs, Id Vd) of transfer and output curves in order to test my simulations. Thanks a lot.

12 February 2015 1,782 5 View

Can anyone help with plant tissue extraction?

I'm looking for protocols for DNA extraction from plants, but they don't talk about the care to be taken when collecting tissues, whether to clean or make any treatment to lower the bacterial load...

02 March 2014 1,985 3 View

What reasons could lead to age of sexual maturity being delayed at high population densities and how do age-specific mortality functions change as density increases?

I am studying density-dependent effects on some life history parameters of a mangrove crab. I found that the size at sexual maturity was delayed at higher density. According to life history...

26 April 2012 8,239 13 View

On the Identification of the X Cation

Hello! I am freshman college student and I really need some enlightenment regarding the identification of cations. The experiment is referred to as 'looking for the unknown' and each is given a...

01 January 1970 7,310 7 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View