Pseudogene EDNRA in gene expression anlysis

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Western blot has no bands after ponceau staining but has marker on membrane?

Hello everyone, I hope you're doing well. Have you ever encountered a situation like this? Recently, I added 30ug/well protein sample and ran the 7.5% gel with 90v 30min (stacking) 130v 70min...

07 November 2023 9,143 3 View

Does someone has the origin code to fit Dielectric permittivity data in Maxwell Wagner model?

My code looks wrong but I don't know how to fix it.

07 May 2023 3,886 0 View

Why don't cells produce cationic lipids?

Positively charged/cationic lipids are widely used as drug vehicles by taking advantage of the non-specific electrostatic binding to the negative surfaces of cells. But if cells bothered to make...

17 June 2021 9,216 3 View

Why are cationic (i.e. lipids) amphiphilics toxic?

Hi all, It is common sense that cationic amphiphiles are toxic. This is true for cationic detergents, lipids and aggregates such as micelles and liposomes. This is also true com complexes with...

12 April 2021 2,470 3 View

How to determine Forster radius of a FRET pair?

Dear all, I'm searching for methods to calculate Forster Radii of FRET pairs, ideally for water-soluble and membrane dyes. Although I am relatively familiar with working with microscopy FRET, I am...

07 December 2020 3,333 3 View

Language teachers' identities influence the implementation of the curriculum. How can we help teachers understand and implement curriculum goals?

In Ontario, language teachers from all grades should prepare students for global citizenship. However, research has shown that for reasons such as lack of training, resources, leadership, etc.,...

02 October 2020 5,719 6 View

How do I measure the fluorescence intensity over time for moving objects?

Hi all, I need to measure the fluorescence intensity for a given object (on two different channels) over time. The object moves (drifts) over time, so its position isn't fixed throughout the...

20 April 2020 3,493 3 View

How can I make input file for rehh package in R with scaffolds?

I am trying to look for positive selection in the genome of a non-model specie, therefore I do not have chromosomes structure, I just have scaffolds and positions of SNPs in a VCF file. I used...

11 March 2020 3,867 3 View

Can supported lipid bilayers be formed with cationic lipids?

Hello there, I am trying to coat plastic beads (spherotech beads) with cationic lipid bilayers. There are several protocols for neutral and negatively-charged bilayer formation on polystyrene...

24 July 2019 8,588 7 View

How can I obtain the meq/kg value of lignocellulosic biomass?

I followed the procedure below in order to analyze the buffering capacity of four different types of biomass. I order to decrease the pH of the liquid to 3, the titration volume (0.01M H2SO4) used...

09 May 2017 5,098 1 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View