How to scale up forest carbon stock assessment from plot scale to regional scale?

The research focuses on the mangrove forest ecosystem. Then it will conduct biomass and carbon stock estimation at the plot scale? How to scale up forest carbon stock assessment from plot scale to...

29 December 2020 1,421 3 View

Get PMID from PubMed automatically?

Dear experts, I got first author, publication year, journal, and paper title in separate columns of a Google Sheet. Are there ways to crawl PubMed and automatically get the PMIDs of the listed...

23 December 2020 3,949 4 View

Should we include 2 clinically correlated variables (Correlation constant = 0.8) in the same Multi-linear regression model?

I am currently studying the effect of atrophy of a muscle on the clinical outcome of joint injury. There is actually another muscle that was previously well established to have an effect on...

06 September 2020 9,986 7 View

How can I extract all-time Twitter contents using keywords, without Premium or Enterprise API?

I would like to analyse Twitter contents, the words in the mentions, the locations of the Tweets, how many re-tweets and favorites, the trending, etc, based on keyword searches. I see that many...

06 April 2020 5,531 5 View

What is the appropriate OD/wavelength (spectrophotometer) for measuring bacterial growth in broth?

Hi all, I am working on growing actinomycetes in broth and to measure its turbidity in indicating the presence of growth. Why are we measuring optical density of bacteria at 600nm in general?...

18 September 2018 1,697 7 View

How can extract required data for Meta-analysis?

May I know how to extract required data for meta-analysis? For example, the inclusion criteria of our review is only limited to participants over 60 years of age. How can we extract data from the...

24 March 2018 7,001 2 View

How to process NGS sequencing data for amplicons from multiplex?

Hi all, I've run 6 rounds multiplex PCR of 21 self-designed microsatellite primers on bear samples. I have pooled all the pcr products, purified the amplicons and sent for NGS sequencing. I have...

12 September 2017 8,241 2 View

Statistic related question

15 June 2017 6,435 2 View

Why there is no bands / smearing observed for genomic DNA samples during gel electrophoresis?

05 September 2016 1,360 13 View

Hello everyone, I am running a experiment on voltammetry and wish to reduce a system?

to the system, I connected reference and working electrode and to a buffer I connected CE. I applied a negative voltage of -350mV. The reduction potential of my system is -325mV. At the start, my...

01 September 2016 7,604 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View