How to Detect Water in THF (Tetrahydrofuran) ?

Hello, we have solvent purifier system in our lab. But There is some unwanted results for our experiment. We are suspecting purity of solvents. Are there any simple methods to detect water in THF?

19 November 2020 114 5 View

If an animal feeds mainly on algae but also eats cyanobacteria and insects, which group does this animal belong to; herbivore or omnivore?

I wonder if a fish (Random sp.) feeds mostly on plankton but also consumes cyanobacteria and some insects (Chironomidae, Simuliidae) at much lower rates, can we classify them as omnivore?

03 March 2020 8,177 4 View

How does the amount of amino acid exceed the amount of crude protein in fish meal (Dry matter basis)?

I read a SCI article where the author analysed several fish meals' amino acid profiles and crude protein levels. I.E., The findings indicate that the crude protein level is 79.50g/100g for fish...

16 July 2019 2,039 6 View

Which one should I choose from the results of a sample with different amino acid analysis methods?

I have a protein source with varying results of amino acid profile. For instance; when the sample is hydrolysed with "6 M HCI" L-Valine level is 0.24g/100g whereas it is 3.08g/100g when...

23 February 2018 5,648 2 View

What is the difference between higher order (second order) confirmatory factor analysis and bifactor analysis?

24 July 2017 5,178 5 View

How to determine total RNA concentration when you have large amount of miRNA?

I am isolating total RNA with miRNeasy micro (PBMCs) miRNeasy serum plasma (serum) and exoRNeasy (serum/exosome) for Next Gen Sequencing for miRNA. I believe my final product should contain mostly...

30 March 2016 3,006 4 View

How can I do a reaction between polyamine and lipoic acid NHS ester in an organic solvent?

I want to do this reaction but as far as learned from literature, NHS ester-amine reaction is done in a phosphate buffer solution between pH 8 to 9. However, lipoic acid modified polymer...

27 June 2015 1,731 3 View

An economic method for the fluorescent labeling of PCR fragments?

I have a trouble with my pcr reactions and i want to ask you where is my fault.I designed six primer pairs according to Schuelke's M13 methods but I did not get any reaction.One of my primers...

23 December 2014 4,623 5 View

What is the reason for different fold changes in gene expression from independent experimental repeats in Real-time PCR experiments ?

25 October 2014 7,228 3 View

How should I calculate a touchdown PCR reaction with a low dna quantity?

14 September 2014 2,091 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View