Hi Researchgate community!

I am trying to optimize using NanoTRAP beads from Chromotek for isolating protein and RNA interactors of my protein of interest.

My bait protein is fused to mNeonGreen (mNG) so I am going to use mNeonGreen TRAP (https://www.ptglab.com/products/mNeonGreen-Trap-Agarose-beads-nta.htm?srsltid=AfmBOoqSBHqj_Xnel7uGeg1o4PNDx4cD0-l2MULhdhYzETHsdPu02cmu)

I have been able to somewhat elute the bait protein from a test run of the beads.

The non-crosslinked lysate seems to elute them better than when the cells are cross linked (0.5% formaldehyde) prior to lysate preparation.

My goals are to:

1) Improve the IP efficiency

2) find a reliable protocol to isolate RNA that is bound to my bait protein.

Thanks in advance!