Protocol for methylene blue staining of bacterial cells to differentiate live and dead cells?

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How to prepare liposome samples for Scanning electron microscopy and transmission electron microscopy?

Liposome sample preparation - TEM and SEM

20 May 2024 3,651 2 View

How much lipid concentration and rehydration solution should be added to form lipid vesicles (DMPC)?

Liposome preparation

17 January 2024 9,326 0 View

During MOF synthesis , pre calculation of organic linkers with metals selection should be done In ab initio method ?

During MOF synthesis, pre-calculation of organic linkers with metals selection should be done In the ab initio method (or any other software) to get the exact product, is that a necessary...

15 January 2024 3,045 2 View

How to prepare liposome sample(MLV,SUV) for SAXS analysis?

Small angle X-ray scattering-Liposome

10 January 2024 9,416 0 View

How to prepare liposome samples(suspended in solution) and form thin film to investigate in SEM and XRD?

Liposome XRD-SEM

02 January 2024 6,803 5 View

What methods can be used to coat liposome samples to ITO slides for XRD analysis?

Liposome-XRD analysis

30 December 2023 8,865 3 View

How to make small unilamellar vesicles from multilamellar vesicles by sonication? at what time/ frequency need to make this SUV?

Lipid Vesicle preparation

28 November 2023 7,405 2 View

What analysis can be done to differentiate multilamellar from unilamellar vesicles?

Liposome

28 November 2023 8,604 4 View

What resistivity ITO slide is suitable for XRD analysis of multilamellar/ unilamellar liposomes?

X-ray diffraction

28 November 2023 3,561 3 View

When PCR product is being used as template, PCR amplification is not happening. What might be the reason?

I have been trying to amplify one of the gene by PCR and I have optimised the conditions for the same and have got an amplicon. To confirm if the amplicon is my desired gene, I am using PCR...

27 October 2023 5,720 18 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Does matter flow through ecosystems and energy is recycled and how does the flow of matter and energy compare in the living world?

03 August 2024 607 2 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...

02 August 2024 8,572 0 View

How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?

01 August 2024 7,061 2 View

How Do Project Data Analytics and AI Advance Quality 4.0 in Construction Project Management?

As the construction industry advances, the integration of Project Data Analytics and Artificial Intelligence (AI) is becoming increasingly crucial in project management. These technologies are...

31 July 2024 6,484 1 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,484 5 View

Jeffrey P Cheng

We use a 1:5 dilution of cell dilution to Trypan Blue (not methylene blue) solution. Blue/purple cells are dead/dying and the clear white cells are alive. there are numerous Trypan Blue protocols available. It is also commercially prepared and available.

Ayshwarya Ravikumar

Jeffrey P Cheng Thank you very much