Dear Ayshwarya Ravikumar ,

you may have a look at the answers of a similar question of the Q&A section of RG:

https://www.researchgate.net/post/Whats_the_best_sample_preparation_to_investigate_liposomes_in_a_TEM

and also the following paper may help:

Chapter Imaging of Liposomes by Transmission Electron Microscopy

See also:

https://www.protochips.com/wp-content/uploads/2017/10/app-drug-delivery.pdf

and the section 'TEM analysis*)' (page 560) in:

Article AFM, ESEM, TEM, and CLSM in liposomal characterization: a co...

*) and references therein

Good luck and

best regards

G.M.

Do you have cryoTEM or room temperature TEM?

For room temperature TEM, you need to do negative staining.

For cryoTEM, you need to plunge-freeze your samples (negative staining is not required).

Before you load samples on the grids (for both cryoTEM and room temperature TEM), perform glow discharge on the grids.