I am looking for a protocol for extracting miRNA and RNA, avoiding the use of a commercial kit.
TRIZOL, will work fine, and the final RNA will have both miRNA and mRNA.
Thanks you very much Julio! But I was also interested in a protocol for purifying and concentrate a miRNAs from cell supernatants.
It is for plants use this... http://www.plantmethods.com/content/7/1/4 its very simple.
Http://www.ncbi.nlm.nih.gov/pubmed/23842901
I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.
02 March 2021 8,156 2 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
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20 February 2021 3,366 5 View
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18 February 2021 3,223 3 View
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17 February 2021 2,896 3 View
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17 February 2021 2,797 2 View
I'd like to know if anyone can provide me with a protocol for traditional catalase enzymatic assay in a 96 well microplate, for cell line and/or C.elegans spectrophotometric measurements. Thanks!
11 February 2021 7,273 3 View
I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...
11 February 2021 1,328 3 View
Hi, everyone Could someone recommend papers about Particle swarm optimization (PSO technique), using MATLAB, please? Thanks in advance
03 February 2021 3,811 6 View
25 January 2021 6,257 1 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View