Protocol for extracting miRNA - can anyone help?

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Julio Cesar Cetrulo Lorenzi

TRIZOL, will work fine, and the final RNA will have both miRNA and mRNA.

José Santiago Ibáñez Cabellos

Thanks you very much Julio! But I was also interested in a protocol for purifying and concentrate a miRNAs from cell supernatants.

Ricardo Ivan Alcala-Briseño

It is for plants use this... http://www.plantmethods.com/content/7/1/4 its very simple.

Oscar Correa Jimenez

Http://www.ncbi.nlm.nih.gov/pubmed/23842901