Hi,

Immunoprecipitation of YFP (your favourite protein), followed by loading on SDS page and detection with Ab@ubiquitin.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149903/

You can also check here before to see if your protein has an ubiquitination site.

http://www.ubpred.org/

Hi,

Immunoprecipitation of YFP (your favourite protein), followed by loading on SDS page and detection with Ab@ubiquitin.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3149903/

You can also check here before to see if your protein has an ubiquitination site.

http://www.ubpred.org/

Hi,

I agree with Francois, Immunoprecipitation followed by WB with anti-Ubiquitin is the most common way, If you have a good anti-ub antibody. In my hands, mouse

monoclonal anti-ubiquitin (clone FK2) from Affiniti Research, worked quite well.

You can treat your cells with proteasome inhibitors (MG132) a few hours before harvesting to increase the probability of detecting ubiquitination. This treatment should also increase the satbility of your protein, which would be an indirect proof that your protein is ubiquitinated,

Good luck

Thanks a lot! I will try!