protein purification-IPTG stability

03 March 2010 1 4K Report

can any tell me how stable is IPTG stock. i have made a stock of 0.5M IPTG one year back, can i use it now

regards

aparna

In LB plates for blue/white screening you can use it at double concentration, but for protein production and purification throw it away. In this case for 2-4h of IPTG induction I use to add directly the powder in the broth because the possibility of protein contamination is negligible

ciao!

Paola

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