Hello, can anyone assist me with research papers on how to identify the cDNA region encoding mature peptides of genes.
Just use any genome browser, I personally use the UCSC one, you will get the genomic, mRNA and protein sequence for the genes you look for.
I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...
15 July 2024 3,977 1 View
Would an average spot welder suffice for welding tabs for pouch cell electrodes instead of an ultrasonic one? Why would an ultrasonic welder be better than a regular spot welder?
10 July 2024 8,763 0 View
The impact im looking for could be both positive and negative on the electric grid
18 June 2024 9,269 3 View
IT
09 June 2024 735 1 View
What are the major ethical issues to be considered while working on design and construction of electronics device in a research work.
03 June 2024 8,189 4 View
The impact of monetary policy and bank rate on the performance of deposit money banks in Nigeria
31 May 2024 7,587 1 View
The result from PlasmidFinder shows that the assembled genome harbored many plasmids. Consequently, I want to investigate whether the plasmids are encoded for ARGs. And determine if they are on...
08 May 2024 7,467 0 View
ls it possible to emerge the wi-fi and Li-fi in one device and regular them.
21 April 2024 9,292 0 View
The code needs to be able to work on a 28-bus system having cost as the objective function and constrain like transmission loss.
18 April 2024 3,349 1 View
I want to understand why most analyses conducted in Africa use GMM instead of other panel data estimation techniques.
16 April 2024 5,322 3 View
We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...
08 August 2024 4,842 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?
31 July 2024 6,544 11 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View