Hello, can anyone assist me with research papers on how to identify the cDNA region encoding mature peptides of genes.
Just use any genome browser, I personally use the UCSC one, you will get the genomic, mRNA and protein sequence for the genes you look for.
In a topology optimization machine learning prediction algorithm, can I consider the OOB score of RF as the same as R^2 score. Could that be a more unbiased way of validating the model
14 February 2021 3,540 1 View
I want to perform a simulation study to examine the consistency property of parameters of a new distribution through the quantile function. The simulation study will contain many replicates (say...
10 February 2021 180 3 View
Is there any recent work available on the performance of P2P Lending in India?
05 January 2021 5,211 3 View
I am working to improve a manuscript and I have been advised to provide a map showing where the correlation is significant. Any information or links to learn about this would be quite helpful....
26 December 2020 1,162 5 View
Water is a molecule that has a very high boiling point compared to ethanol. Why does water have such a high boiling point compared to a molecule like ethanol, and what are the...
23 November 2020 9,662 8 View
I am looking for a website of blog where i can get details about the relevant datasets used for question answering in the medical field. What i found, are not explaining in details these...
10 November 2020 7,202 3 View
Can anyone guide me to some recent studies on Impact of Microfinance in India on Poverty, Employment and Incremental Income. Many Thanks.
09 October 2020 9,962 1 View
What Economic Activities taken up with Microfinance loans can yield returns greater than 25% which is the usual cost of a micro loan in India?
09 October 2020 3,777 5 View
I will be extracting trypanosome DNA from mid-guts of tsetse flies for LAMP analysis. Kindly advice on protocol to adopt for maximum yield and benefits of Chelex method over the regular method....
23 September 2020 504 4 View
I obtained the nodal displacement values of (u1,u2,u3) from a bar in an analysis. I want to use these spatial displacement values as the boundary conditions for the undeformed instance of the bar...
17 September 2020 7,793 3 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View